MEMS-Based Microdevice for Cell Lysis and DNA Extraction
نویسندگان
چکیده
منابع مشابه
ONE-STEP NUCLEIC ACID EXTRACTION AND AMPLIFICATION ON AN INTEGRATED MICRODEVICE FOR EARLY DETECTION OF Cochlodinium polykrikoides
Herein, a monolithic polycarbonate (PC) microdevice was developed to handle both DNA extraction and polymerase chain reaction (PCR) processes successively. Target cell – Cochlodinium polykrikoides microalgae, the causative species for harmful algal blooms in South Korea – was firstly captured on amine-functionalized microbeads via electrostatic interaction and then lysed to release DNA into sub...
متن کاملMiniaturized bead-beating device to automate full DNA sample preparation processes for gram-positive bacteria.
We have developed a miniaturized bead-beating device to automate nucleic acids extraction from Gram-positive bacteria for molecular diagnostics. The microfluidic device was fabricated by sandwiching a monolithic flexible polydimethylsiloxane (PDMS) membrane between two glass wafers (i.e., glass-PDMS-glass), which acted as an actuator for bead collision via its pneumatic vibration without additi...
متن کاملMicrofluidic extraction, stretching and analysis of human chromosomal DNA from single cells.
We describe a microfluidic device for the extraction, purification and stretching of human chromosomal DNA from single cells. A two-dimensional array of micropillars in a microfluidic polydimethylsiloxane channel was designed to capture a single human cell. Megabase-long DNA strands released from the cell upon lysis are trapped in the micropillar array and stretched under optimal hydrodynamic f...
متن کاملAn efficient and cost-effective method for DNA extraction from athalassohaline soil using a newly formulated cell extraction buffer
The present study describes the rapid and efficient indirect lysis method for environmental DNA extraction from athalassohaline soil by newly formulated cell extraction buffer. The available methods are mostly based on direct lysis which leads to DNA shearing and co-extraction of extra cellular DNA that influences the community and functional analysis. Moreover, during extraction of DNA by dire...
متن کاملFull Three-Dimensional Motion Characterization of a Gimballed Electrostatic Microactuator
Advanced testing methods for the dynamics of microdevices are necessary to develop reliable marketable microelectromechanical systems (MEMS). The main purpose for MEMS testing is to provide feedback to the design-and-simulation process in an engineering development effort. This feedback should include device behavior, system parameters, and material properties. An essential part of a more effec...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2017